tlr2 antibody (11g5) - azide free  (Bio-Techne corporation)

Journal: Cell reports

Article Title: Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer

doi: 10.1016/j.celrep.2022.111596

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-TLR2 , Novus Bio , Cat #NBP2-24861; RRID:AB_2924685.

Techniques: Plasmid Preparation, Polymer, Staining, Virus, Recombinant, Sequencing, Software

Journal: Frontiers in microbiology

Article Title: Human herpesviruses-encoded dUTPases: a family of proteins that modulate dendritic cell function and innate immunity.

doi: 10.3389/fmicb.2014.00504

Figure Lengend Snippet: FIGURE 3 | Activation of NF-κB by herpesviruses-encoded dUTPases is weaker in TLR2/TLR6-HEK293 than in TLR2/TLR1-HEK293 expressing cells. Herpesviruses-encoded dUTPases mediated activation of NF-κB in (A) TLR2-HEK293 and (B) TLR2/TLR6-HEK293 cell lines. Cells were transiently transfected with NF-κB luciferase and pRL-TK reporter plasmids and co-transfected with empty vector, as described in Materials and Methods. Empty vector was used as a carrier to keep the total amount of transfected-DNA constant. After 24–36 h, cells were treated with various herpesviruses-encoded dUTPases (10 μg/ml) or left untreated for 8 h, and NF-κB luciferase levels were measured. Zymosan (10 μg/ml) and FSL-1 (0.1 μg/ml) were used as positive controls for TLR2 and TLR6 activation, respectively. Data were normalized for transfection efficiency by measuring Renilla luciferase activity and expressed as the mean fold induction ± SD relative to control levels. Values represent the average of three independent experiments.

Article Snippet: IgG2a Isotype control monoclonal antibody was purchased from eBioscience (San Diego, California) and anti-TLR2 (clone TL2.1) monoclonal antibody was purchased from Imgenex (San Diego, California).

Techniques: Activation Assay, Expressing, Transfection, Luciferase, Plasmid Preparation, Activity Assay, Control

Journal: Frontiers in microbiology

Article Title: Human herpesviruses-encoded dUTPases: a family of proteins that modulate dendritic cell function and innate immunity.

doi: 10.3389/fmicb.2014.00504

Figure Lengend Snippet: FIGURE 4 | Herpesviruses-encoded dUTPases signal through TLR2/TLR1 and MyD88. TLR2- HEK293 cells were transiently transfected with NF-κB luciferase and pRL-TK reporter plasmids and co-transfected with pCMV-TLR1 and either TLR2DN, a vector that expresses a dominant-negative form of TLR2, or empty vector (0.3 μg) (A). After 24–36 h, cells were treated with the dUTPases (10 μg/ml) encoded by HHV-6A, HHV-8 and VZV or left untreated for 8 h, and luciferase reporter gene activity was measured. Pam3Csk4 (0.1 μg/ml) was used as a positive control for TLR2/TLR1 activation and nuclear human dUTPase was used as a control protein. (B) Anti-TLR2 blocking Ab inhibits NF-κB activation by the herpesviruses-encoded dUTPases. TLR2-HEK293 cells were transiently (Continued)

Article Snippet: IgG2a Isotype control monoclonal antibody was purchased from eBioscience (San Diego, California) and anti-TLR2 (clone TL2.1) monoclonal antibody was purchased from Imgenex (San Diego, California).

Techniques: Transfection, Luciferase, Plasmid Preparation, Dominant Negative Mutation, Activity Assay, Positive Control, Activation Assay, Control, Blocking Assay